Derya EGELI YILMAZ, Gizem TIRIS, Şerife Evrim KEPEKCI TEKKELI
Journal of Research in Pharmacy - 2026;30(3):826-834
This study introduces a combined HPLC and UV detection technique for the quantification of casticin in capsule and human plasma samples. The chromatographic separation was carried out utilizing a C18 column (150 mm x 4.6 mm x 5 mum) at a temperature of 25 º C. Isocratic elution with a mobile phase comprising 60:40 v/v (methanol -0.05% formic acid) was employed. Flow rate was adjusted 1 mL/min. The analyte was determined at a wavelength of 258 nm, with a retention time of 14.7+/-0.01 min. The developed method underwent validation according to ICH criteria, covering specificity, linearity, precision, accuracy, detection and quantitation limits, as well as robustness. The linear range was determined to be 10 -60 ng/mL for both capsule and spiked plasma specimens. The suggested technique was performed to the analysis of casticin in spiked human plasma and pharmaceutical preparations, yielding a recovery of 106.04% and demonstrating precision through intra -day and inter -day experiments with the highest relative standard deviation (RSD %) value of 4.94. Consequently, the technique was performed to the quantifying of human plasma specimens from in a patient taking medication containing casticin.
