GÜLŞAH BALKAN, BURAK COBAN, KASIM C GÜVEN
Acta Pharmaceutica Sciencia - 2005;47(2):93-106
Agarose and Gracilaria verrucosa agar were fractionated by Sephadex G-25 and SPE columns (butyl, octadecyl, alumina and quaternary amine). IR spectrophotometric and metachromatic methods were used for the identification of the fractions. In contrary to findings of Tsuchiya and Hong, (1965) IR spectrum of agar and agarose were not the same. Agar showed different IR bands than agarose as at 505, 516, 534, 580, 617, 667 cm-1. Agar was not unique compound, after fraction various agars gave different IR spectra. However agar and agarose gave the same metachromatic β band. Hence differenciation of agar and agarose is possible by IR spectra but not metachromatic method.
