PRACHİE SHARMA, KAPİLA KUMAR, KAMAL RAWAL
Journal of Research in Pharmacy - 2022;26(3):483-493
Hepatitis B virus (HBV) is the predominant cause for the liver-related malignancies worldwide. Recently, clathrin mediated pathway has been found to assist the Hepatitis B virion in entering the host cell successfully. The role of the viral L protein subunit (LHB) has been understood to interact with clathrin proteins during the clathrin mediated endocytosis. The role of the viral X protein is necessitated here owing to the regulatory nature of this protein during the pathogenesis, which however has been quite elusive. Therefore, an in-silico study has been designed to assess the interaction possibilities between the adaptor protein Gamma 2 adaptin and the regulatory viral protein X (HBX). This study is designed to predict the interaction between Viral HBx protein and human Gamma 2 adaptin protein of clathrin mediated endocytic pathway during the Hepatitis B viral infection using insilico protein protein docking tools. The protein complexes Gamma-2-Adaptin-HBx and Gamma-2-Adaptin-LHB were then docked using Cluspro, Hex and HDock respectively. We found that Gamma-2-Adaptin-HBx binding energies using Cluspro and hex we more negative as compared to the ones obtained for the Gamma-2-Adaptin-LHB complex. This study indicates towards the HBX viral protein in being on of the important interactors of Gamma 2 adaptin in addition to LHB.
