ELİF GÜZEL, MUKHLESUR RAHMAN
Acta Pharmaceutica Sciencia - 2025;63(3):532-547
Hypericum perforatum L. has been used for centuries as a herbal remedy against variety of diseases for its biological activities. It has been also used as an antibacterial agent against different bacteria. The aim of this study is to investigate the antibacterial properties of the H. perforatum L. extracts against several clinical isolates and explore their chemical composition. In terms of S. aureus strains, n-hexane extract demonstrated the best activity against XU212 strain, with the MIC of 256 µL/mL. The MIC was 512 µL/mL for other S. aureus strains for n-hexane and dichloromethane extracts. n-hexane extract demonstrated activity against E. coli ATCC 25922 with the MIC of 1 µL/mL. UPLC was performed for dichloromethane and methanol extracts. The main compounds were identified as catechin, hyperforin, and rutin from methanol extract, and hypericin and luteoskyrin from dichloromethane extract. S. aureus demonstrated good antibacterial activity against tested MRSA and Bacillus subtilis strains.
