Hasan ARSLANYÜREĞİ, Melike ERSÖZ, Tuncay ALTUĞ
Demiroğlu Bilim Üniversitesi Florence Nightingale Tıp Dergisi - 2025;11(3):144-149
Objectives: In the present study, epigallocatechin-gallate (EGCG) was investigated for its ability to induce programmed cell death in rat-derived C6 glioma cells. Materials and methods: The C6 glioma cells were cultured. Then the cells were incubated with 0 (Control), 50, and 100 mug/mL EGCG for 24, 48, and 72 h. After the treatment of EGCG, the apoptotic cell death was assayed using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method. The data obtained were analyzed using One-Way ANOVA. Results: The study determined that EGCG, at concentrations of 50 and 100 mug/mL, induced apoptosis in C6 glioma cells after 24, 48, and 72 h. The most significant increase in apoptosis was observed in cells treated with 100 mug/mL EGCG for 72 h (p<0.001). Conclusion: The present study demonstrates the apoptotic effect of EGCG on tumor cells, as assessed by TUNEL, supporting its potential as an alternative bioactive compound in cancer biology and therapeutic research. Given the molecular mechanisms of EGCG, combined treatment strategies for cancer therapies may be developed.
