Sathish SABBANI, Chakravarthi GUNTUPALLI, Narender MALOTHU
Journal of Research in Pharmacy - 2026;30(3):868-881
In this work, the amount of Ritlecitinib in rat plasma was determined using a liquid chromatography - tandem mass spectrometry approach. Crizotinib is an internal standard(IS). With a 3.5µm particle size and dimensions of 150 x 4.6 mm, the Waters X -Terra RP -18 column was used for the chromatographic separation. Acetonitrile: TEA pH - 2.5/Formic acid in a 60:40% v/v ratio was used as the mobile phase at a flow rate of 1.0 mL/min. The drug and IS were extracted using the liquid -liquid extraction technique. Proton adducts of Ritlecitinib and Crizotinib were detected at m/z 286.159 -105.7458 and 451.3378 -282.4251, respectively, in the positive mode of multiple reaction monitoring (MRM). The validation was carried out within the linear concentration range of 12.50 -100.0 ng/mL. The coefficient of variation (%CV) for Ritlecitinib was determined to be 2.80% and 96.47%. Further, the precision and accuracy results for HQC, MQC, LQC, and LLQC were determined to be 98.02%, 98.42%, 98.62%, and 96.13%. With this method the average recovery rate was found to be 98.78 %.
