İLHAN SABANCILAR MURAT AYDEMİR SEÇKİN KAYA
Northwestern Medical Journal - 2025;5(2):112-121
Aim: To determine the anticancer and antioxidant activity levels of the synthesized heterocyclic molecule named 1-benzyl-1-(2- methyl-3-oxo-3-(p-tolyl)propyl) piperidin-1-ium chloride. METHODS: The molecule 1-benzyl-1-(2-methyl-3-oxo-3-(p-tolyl) propyl)piperidin-1-ium chloride was synthesized solvent-free via microwave synthesis. Piperidine purification involved dichloromethane extraction with 2 M HCl, followed by 5% NaHCO3 and precipitation with n-hexane. Anticancer activity on A549 lung cancer cells was assessed using the MTT assay. Antioxidant activity was evaluated by DPPH and CUPRAC methods at five concentrations (250-15.6 µM), with ascorbic acid as a control. Results: The heterocyclic molecule dissolved in PBS was tested for anticancer activity on A549 cells at concentrations ranging from 6.25 to 100 µM. Cytotoxicity was highest at 66.90% for 100 µM and decreased to 5.57% at 6.25 µM, with an IC50 of 32.43 µM. In DPPH assays, the absorbance for AscA varied from 1.263±0.057 to 0.675±0.093, while the piperidine molecule ranged from 1.339±0.044 to 1.072±0.120. In CUPRAC assays, AscA absorbance was 0.227±0.052 and 1.768±0.176, and for the piperidine molecule, it was 0.132±0.042 and 0.142±0.031. Conclusion: Piperidine is considered a saturated heterocyclic ring and possesses a wide range of biological activities. In this study, it was observed that the synthesized piperidine molecule showed limited DPPH radical scavenging activity. It also showed a high level of cytotoxic effect on A549 cancer cells and could be an important molecule for anticancer studies.
