Farzana SADAF, Muhammad AZAM, Syed Waleed Ahmed BOKHARI, Sabiha GUL, Sumbul SHAMIM
Journal of Basic and Clinical Health Sciences - 2026;10(3):305-317
Purpose: To compare the antihyperlipidemic effects of Garlina and Murraya koenigii (MURK) extracts in hyperlipidemic rats. Material and Methods: Wistar rats were fed a high-cholesterol diet (HCD) and treated orally for 60 days with Garlina (50 mg/kg) or sequential extracts of Murraya koenigii leaves prepared using solvents of increasing polarity: hexane (MURK-HEX), ethyl acetate (MURK-EA), and methanol (MURK-MeOH). Atorvastatin served as the positive control. Body weight, food intake, and serum and tissue lipid profiles were evaluated. Data are expressed as mean +/- SEM (n = 7) and analyzed using ANOVA followed by Games-Howell or Bonferroni post-hoc tests comparisons against HCD rats. Results: Two-way ANOVA revealed significant effects of treatment, time, and their interaction on food intake, with all treated groups showing marked reductions versus HCD (p < 0.001). MURK-HEX produced the greatest reduction in body weight and food intake. Lipid analysis showed that MURK-EA resulted in the lowest hepatic cholesterol, while MURK-MeOH significantly reduced aortic cholesterol and triacylglycerol levels in both liver and aorta. Serum lipid profiling revealed that MURK-MeOH produced the lowest triacylglycerol, LDL, and total cholesterol levels. Atherogenic index of plasma (AIP) showed an inverse relationship with percentage protection across all treatment groups. Conclusion: Both Garlina and Murraya koenigii extracts exhibited significant lipid-lowering effects. Garlina achieved the lowest AIP, comparable to atorvastatin, likely due to enhanced HDL levels.
