Neelam MITTAL, Harakh Chand BARANWAL, Burugupalli SWETHA, Vijul VIJUL, Mohammad Faisal AZEEZ, Gracemol RAJU
European Endodontic Journal - 2026;11(1):1-8
Aim: This randomised clinical study assessed and compared the healing outcomes, both clinical and radiographic, of platelet-rich fibrin (PRF) and human amniotic membrane (HAM) scaffolds in regenerative endodontic therapy for necrotic mature permanent teeth with apical periodontitis. Methodology: Thirty participants presenting with necrotic mature permanent teeth and apical lesions were assigned to either the PRF or HAM group (n = 15 each) randomly. Follow-up evaluations were performed at baseline, 6 months, and 12 months. Results: At the 12-month follow-up, both groups demonstrated favourable clinical outcomes, with resolution of symptoms and radiographic evidence of periapical healing. The CBCT analysis revealed significant reductions in lesion volume for both PRF (65.26%) and HAM (64.75%) groups (P < .005), without a statistically significant difference between the groups. Conclusion: Both PRF and HAM scaffolds supported periapical healing in necrotic mature permanent teeth. However, biological regeneration of the functional pulp-dentin complex could not be confirmed, indicating that periapical healing occurred without verified neurovascular re-establishment.
