Ezgi Nur ÇİL, Yasemin SOYSAL
Journal of Basic and Clinical Health Sciences - 2026;10(3):397-402
Purpose: SIRT1 functions in regulatory mechanisms including cellular aging, apoptosis, glucose and lipid metabolism, oxidative stress and inflammation. Studies show that SIRT1 inhibitors may affect various types of cancer and metabolic disorders, making SIRT1 an important therapeutic target. Boric acid is an inorganic boron compound with antimicrobial, antifungal and potential anticancer effects. Calcium fructoborate, another boron compound found in vegetables and fruits, has anti-inflammatory, antioxidant and anti-tumor effects. This study aimed to investigate the inhibitory potential of calcium fructoborate on SIRT1 activity and to evaluate its possible use as a chemical tool for future studies targeting SIRT1 regulation. Material and Methods: In this study, the effect of calcium fructoborate on SIRT1 activity was investigated by a fluorescence-based method using human recombinant SIRT1. Results: Calcium fructoborate inhibited SIRT1 activity in a concentration-dependent manner. Calcium fructoborate significantly inhibited SIRT1 activity by 42% at a concentration of 20 mM (p < 0.001). Boric acid did not produce a statistically significant or consistent concentration-dependent change in SIRT1 activity within the tested concentration range. Conclusion: These preliminary cell-free findings suggest that calcium fructoborate inhibits SIRT1 activity, indicating its potential as a chemical tool for studying the biological functions of SIRT1. However, the biological relevance of this effect and its potential therapeutic implications require confirmation through further studies in cellular and in vivo models.
