AYESHA ASIF, M ASİF SAEED
Acta Pharmaceutica Sciencia - 2021;59(1):615-630
Cytolysin potential of the constituents of Guilandina bonducella seeds was evaluated. For this purpose, di and triterpenes had been investigated. Two di and four triterpenes from the EtOAc extract of G. bonducella seeds were isolated, purified by chromatographic and re-crystallization methods and identified by comparative spectroscopic data. Cytolysin potential of these compounds was carried out on brine shrimp’s (Artemia salina) larvae (nauplii), compared with positive controlled colchicine’s reaction and evaluated by LD50. Diterpenes (neocaesalpin C, neocaesalpin D) and triterpenes (α-amyrin, β-amyrin, lupeol and lupeol acetate) exhibited a marked cytolytic reaction, even though their intensities differ from each other and with the colchicine. EtOAc extract of G. bonducella seeds contained diterpenes (neocaesalpin-C and neocaesalpin-D) and triterpenes (α-amyrin, β-amyrin, lupeol and lupeol acetate). These compounds had cytotoxic ability to the brine shrimp’s larvae.
