Bircan KOLBAŞI ERKAN, Talat Yasin ACAR, Muhammet Volkan BÜLBÜL, Azat POLAT, Kaan SAPMAZ, İlknur KESKİN
Clinical and Experimental Health Sciences - 2026;16(1):1-9
Objective: Metastasis, a critical process in cancer progression, is mediated predominantly by integrins, including the alphavbeta3 integrin, which plays a key role in cellular adhesion, migration, and survival. Both cell lines have significant metastatic potential, and alphavbeta3 integrin plays a role in their interaction with the extracellular matrix and invasiveness. This study investigated the antiproliferative potential of phenylboronic acid in SK-OV-3 ovarian adenocarcinoma and MIA-Pa-Ca-2 pancreatic carcinoma cell lines and its effects on integrin alphavbeta3 expression. Methods: SK-OV-3 and MIA-Pa-Ca-2 cells were cultured under standard conditions. Cells were treated with phenylboronic acid concentrations of 10-500 muM for cell viability analysis (MTT) and 50, 100, 500 muM for immunofluorescence staining analyses. An immunofluorescence assay was performed to assess alphavbeta3 integrin expression levels, and fluorescence intensity was analyzed as a surrogate marker of protein expression. Results: MTT analysis showed that the IC?? values of PBA after 24 h exposure were 264.8 muM for SK-OV-3 and 294.4 muM for MIA-Pa-Ca-2 cells. Immunofluorescence revealed a dose-dependent decrease in alphavbeta3 integrin intensity in both cell lines (p<.0001). In SK-OV-3 cells, 50 muM PBA reduced fluorescence to ~45% of control, whereas MIA-Pa-Ca-2 required 100-500 muM for similar effects. Conclusion: This study demonstrated that phenylboronic acid exerts a dose-dependent antiproliferative effect in SK-OV-3 and MIA-Pa-Ca-2 cells and is associated with a concentration-dependent reduction in alphavbeta3 immunofluorescence signal, particularly at lower doses in SK-OV-3 cells. Given the well-described role of alphavbeta3 in cell adhesion, migration and metastatic spread, these observations raise the possibility that PBA may influence metastasis-related pathways, a hypothesis that should be tested in future functional studies.
