AYŞEGÜL BERKTAŞ, ELİF NAZ GÜRSOY, KAAN KALTALIOĞLU, ŞULE COŞKUN CEVHER
Gazi Medical Journal - 2025;36(2):152-161
OBJECTIVE Diabetes, a significant public health issue today, causes serious health problems due to its complications and will become a global epidemic if no action is taken. Diabetic patients experience elevated oxidative stress, which impairs wound healing. The purpose of this study was to investigate the effects of ellagic acid (EA) and carnosic acid (CA) on oxidative events in liver tissue in diabetic rats with wounds. METHODS The rats were divided into 7 groups as control, untreated-3 day, untreated-7 day, topical-3 day, topical-7 day, oral-3 day and oral-7 day. To induce diabetes in the subjects, each group received a single intraperitoneal injection of streptozotocin. Rats were treated topically or orally with EA + CA. On the 3rd and 7th days of recovery, the rats were sacrificed and the levels of nitric oxide (NOx), malondialdehyde (MDA), glutathione (GSH), protein carbonyls (PC), and ascorbic acid (AA) were measured spectrophotometrically to investigate the effects of oxidative stress in liver tissue. RESULTS Liver tissue MDA, NOx, and PC levels were determined to be statistically decreased in both topical and oral applications compared to the control and untreated groups (p<0.05). Liver tissue GSH, AA, and collagen levels were found to be statistically increased in both topical and oral applications when compared to the control and untreated groups (p<0.05). CONCLUSION These results show that the combination of EA and CA with two different application methods significantly reduces oxidative stress associated with drug metabolism by affecting oxidative events in liver tissue, and has the potential to prevent possible complications of diabetes.
