Fatma AKAT, Eissa ALMAGHREBI, Hüsamettin VATANSEV
International Journal of Traditional and Complementary Medicine Research - 2025;6(3):151-163
Background and Objectives : Lung cancer is the leading cause of cancer death worldwide. Resveratrol (RSV), also known as 3,4',5 -trihydroxy -trans -stilbene, is a phytoalexin and a natural non -flavonoid polyphenol. RSV modulates molecular pathways by influencing Asymmetric Dimethylarginine (ADMA) levels. It also affects key factors critical to lung cancer progression, such as Heat Shock Protein -90 (Hsp -90). The study aims to investigate the possible modulation of Hsp -90 by RSV and its correlation with ADMA levels. Materials and Methods: A549 and HEK -293 cell lines were treated with different concentrations of RSV. The expression levels of Hsp -90 and ADMA were evaluated using ELISA and LC -MS/MS, respectively. Cell viability and proliferation assays were performed using the MTT method to evaluate the effect of RSV treatment on cancer cell growth. Results: RSV suppressed cell proliferation in a dose -dependent manner, highlighting its potential as an anticancer agent. Significant changes in Hsp -90 levels wer e observed in groups treated with 45 µM RSV (p < 0.05). This further supports RSV's potential as an anticancer agent. The study also showed RSV's influence on ADMA levels in A549 cells. The timing of treatment was crucial in determining the extent of the e ffect. Conclusions: This research has contributed to our understanding of the interaction between Hsp -90 and ADMA. It provides insights into the molecular pathways through which RSV exerts its anticancer effects. These findings underscore the need for more research to fully understand RSV's clinical effects and its potential as a lung cancer therapy.
