FARHANA AFROZ, FATEMA MONI, SURİYA SHARMIN, SATYAJİT ROY RONY, FARHANA AFROZ, SHAH MASUM, HOSSAİN SOHRAB
Acta Pharmaceutica Sciencia - 2022;60(3):273-293
Biological samples contain many intrinsic and extrinsic compounds in different concentrations which makes it very challenging to analyze it and cannot normally be injected directly into the analyzing system without sample preparation. Two different sample extraction procedures liquid-liquid extraction and protein precipitation have been employed in this study to quantify fexofenadine in human serum and the recovery rates have been compared. Protein precipitation by methanol has an advantage over liquid-liquid extraction with recovery rates of more than 90% but has limitations due to rise of column back pressure. Due to better recovery rate and quick sample preparation technique the protein precipitation method has been chosen for extraction of drug from serum sample. The developed HPLC method was validated and found to be accurate, precise and specific within the linearity range of 0.8- 4.0 µg/mL. Lower limits of detection and quantification were established as 0.6 and 0.8 µg/mL respectively.
