Rachmi Fanani Hakim, Fakhrurrazi Fakhrurrazi, Anisa Deria, Nindytha Halidzya Feba, Siti Lola Istiqamah, Nabilah Amalia
European Journal of General Dentistry - 2026;15(2):248-255
Objective This study evaluated the oral wound healing ef ficacy and fibroblast proliferation induced by sulfonated Vannamei shrimp chitosan, compared with commercial chitosan. Additionally, its antimi crobial activity against oral microorgan- isms Streptococcus mutans, Staphylococcus aureus, Aggregatibacter actinomycetemcomitans,a n d Porphyromonas gingivalis was assessed. Materials and Methods In the wound healing study, three groups of rats were treated with Aquades (negative control), commercial c hitosan (positive control), and sulfonat- ed shrimp chitosan, with healing time and fibroblast proliferation measured. The antimi crobial study tested different concentrations (1, 3, 5, and 7%) using a diskdiffusion method. Results Sulfonated shrimp chitosan showed the fastest wound healing (5 /C60.47 days) compared with commercial chitosan (6 /C60.0 days) and Aquades (6.6 /C60.6 days) (p¼0.036), and signi ficantly higher fibroblast counts (20.32 /C60.688, p<0.001). Antimicrobial activity was moderate, with the highest inhibition at 3 or 5%. The ANOVA(analysis of variance) test was used for hypothesis testing with the signi ficance level at p <0.05, followed by a Kruskal -Wallis test. Discussion Sulfonated chitosan demonstrated superior wound healing properties, consistent with studies showing enhanced tissue regeneration by sulfonated chitosan derivatives. Antimicrobial effects were moderate, with peak activity at intermediate concentrations and a decrease at 7%, possibly due to a saturation effect.Conclusion These findings suggest sulfonated shrimp chitosan could be a promising agent in wound healing and infection control.
