Selen Şen, Gözde Çakırsoy Çakar, Nur Kazan, Mustafa Kösem
Kastamonu Medical Journal - 2025;5(4):272-277
Aims: This study aimed to determine the expressions of TGR5 (G protein-coupled bile acid receptor 1) and NADPH oxidase 5 (NOX5) in renal cancer subtypes. Methods: In formalin-fixed, paraffin-embedded tissue sections of renal tumor subtypes, TGR5 and NOX5 protein expressions were measured by immunohistochemical staining and mRNA expressions were measured by RT-PCR. Results: Our results showed that TGR5 and NOX5 exhibited differences in their expression levels in renal cell carcinoma (RCC) subtypes, and that TGR5 expression was upregulated in papillary RCC (r=0.53, p<0.01). A positive correlation of TGR5 with NOX5 was detected in the same subtype (r=0.49, p<0.01). After COCOR analysis, a statistically significant difference was found in the TGR5-NOX5 correlation between papillary RCC and clear cell RCC subtypes (z=1.92, p=0.02). At the same time, expression profiles showed that TGR5 was highly expressed in all groups except clear cell RCC compared to normal kidney tissue, but among these, TGR5 had the diagnostic power to distinguish papillary RCC from other groups (AUC=0.94, 95% CI: 0.64-0.95, p=0.005). Conclusion: The present findings indicate the potential of TGR5 as a diagnostic biomarker for papillary RCC, while suggesting that it may have a functional role in regulating the tumor microenvironment together with NOX5.
