Şara Alagöz, Şehnaz Doğa Kök
Demiroğlu Bilim Üniversitesi Florence Nightingale Tıp Dergisi - 2025;11(2):83-87
Objectives: This study explores the interaction between HOX transcript antisense RNA (HOTAIR) and miR-29b-3p across three different breast cancer subtypes, aiming to identify new potential therapeutic targets. Materials and methods: Quantitative polymerase chain reaction was used to analyze the expression levels of HOTAIR and miR-29b-3p in three breast cancer cell lines: MCF-7 (estrogen receptor-positive: ER+), MDA-MB-453 (human epidermal growth factor receptor 2-positive: HER2+), and MDA-MB-231 (triple-negative breast cancer: TNBC). MCF-10A cells were used as the normal control. GAPDH and U6 served as housekeeping genes. Gene expression was calculated using the 2-??Ct method, with statistical significance defined as p<0.05. Results: In MCF-7 cells, both HOTAIR (0.48-fold) and miR-29b-3p (0.18-fold) were significantly downregulated (p<0.05). In MDA-MB-231 cells, HOTAIR expression was moderately reduced (0.82-fold), while miR-29b-3p was increased (1.87-fold), although not statistically significant (p>0.05). In HER2+ MDA-MB-453 cells, HOTAIR was slightly elevated (1.06-fold) and miR-29b-3p modestly decreased (0.96-fold), also without statistical significance. These findings highlight subtype-specific differences in the HOTAIR/miR-29b-3p axis. Conclusion: Our study reveals differential regulation of the HOTAIR/miR-29b-3p axis across breast cancer subtypes, suggesting its potential role in shaping tumor progression and therapy resistance. Findings reveal variable regulation of the HOTAIR/miR-29b-3p axis in breast cancer, highlighting its potential for biomarker and targeted therapy development in TNBC. Further validation with clinical datasets is recommended.
