AİSYAH AL MUMTAHANAH, NAİLA AİSYAH BANOWATI, TARİS ARUNG SAMUDRA DEWI, FARİKHA AMALİNA NURBAITI, SALMA AQİLAH RACHMADANI, AHMAD AINUROFIQ
Acta Pharmaceutica Sciencia - 2024;62(3):631-646
Empirically, snakehead fish with its high albumin content has been widely used in the healing of post-caesarean wounds. Lemon can also be used to prevent wound infections by inhibiting the growth of bacterial activities. This study was conducted to discover the best nanoemulgel formula from the combination of snakehead fish and lemon extract, then evaluate its healing activity for postcaesarean wounds. Nanoemulgel was characterized by pH, viscosity, spreadability, adhesion, particle size, and zeta potential to determine the best formula. Healing activity evaluation was performed on female Wistar rats which were divided into seven groups (n=3). Healing parameters evaluated were wound length closure, epidermal thickening, and tissue reconstruction. Wound length was measured every 5 days for 15 days. Subsequently, histopathological observations H&E staining were used to determine epidermal thickening and wound tissue reconstruction. The results showed that nanoemulgel I (NEG1) containing 7% snakehead fish extract, and 3% lemon extract had the best wound healing ability, with an average wound closure of 84±2.1% on day 10, epidermal thickening of 2.31 ± 0.06 ?m on day 15, and better tissue structure reconstruction than other groups. Thus, the nanoemulgel design can optimize the snakehead fish and lemon extract in accelerating the healing process of post- caesarean wounds.
