Hüseyin Uğur ÖZKAYA, Hasan Anıl KURT, Yasemen Adalı RUŞEN, Emre Onur GÜVEN, Karolin YANAR, Pınar ATUKEREN, Cabir ALAN
Journal of Urological Surgery - 2026;13(3):220-226
Objective: We aim to investigate cold ischemic injury in the kidney harvested from rats and the protective effect of ozone (O3) on cold ischemia damage. Materials and Methods: Thirty Wistar albino male rats were randomly assigned to three groups. Group 1 received isotonic sodium chloride (n=10; I), Group 2 received the University of Wisconsin Solution (n=10; UW), from the aorta during the procedure. Group 3 (n=10; IP + UW) was given O3-oxygen mixture at a dose of 0.7 mg/kg (50 mug/mL) intraperitoneally 30 minutes before the procedure, in addition to the UW Solution. The organs were excised and stored in the given solutions for 12 hours. Thereafter, the kidneys were removed for examination. Results: The biochemical evaluation revealed significantly lower levels of advanced oxidation protein products and protein carbonyl groups in the IP + UW group than in the I group. Statistically, these general protein oxidation indicators were also lower in the IP + UW group compared to the UW group. Superoxide dismutase levels were significantly higher in the IP + UW group than in the UW group. Histopathologically, the number of degenerated or necrotic tubules (D/NT), the number of tubules exhibiting brush border loss (BBT), and the number of tubular cast structures were significantly higher in group I than the UW and IP + UW groups. Comparison of the UW and IP + UW groups showed that the D/NT and the BBT counts were significantly higher in the UW group. Conclusion: Intraperitoneal O3 therapy, together with UW Solution, effectively attenuated cold ischemic injury in rat kidneys.
