İlayda Manav, Selen Güneş, Emre Vural, Şeyma Eroğlu, Elif Giçi, Ayşegül Yıldız
Demiroğlu Bilim Üniversitesi Florence Nightingale Tıp Dergisi - 2025;11(2):67-73
Objectives: This study aims to evaluate the effects of poly(2-hydroxyethyl methacrylate) [p(HEMA)]-based nanoparticles loaded with hyaluronic acid (HA) on the SH-SY5Y human neuroblastoma cell line. Materials and methods: Three experimental groups were established: free HA, unloaded p(HEMA), and HA-loaded p(HEMA) nanoparticles. Treatments were applied for 12 and 24 h. Cell viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, which measures metabolic activity, and the resulting absorbance values were comparatively analyzed. Results: According to the results, HA-loaded p(HEMA) nanoparticles did not exhibit a negative impact on cell metabolism at 12 h; however, a reduction in viability was observed at 24 h. These findings imply that prolonged release may contribute to cytotoxic effects. In contrast, free HA was found to exert a proliferative effect on the cells. For the unloaded p(HEMA) group, an initial decrease in cell viability was observed, which appeared to diminish over time. Conclusion: These findings indicate that HA-containing carrier systems may elicit time- and content-dependent cellular responses and hold potential for neurological targeting.
