MURAT DOĞAN
Türk Fen ve Sağlık Dergisi - 2022;3(3):236-245
Purpose: In this study, it is aimed to prepare chitosan nanoparticles including shRNA-VEGF and evaluate their bioactivity by in vitro cell culture studies and to perform mechanical characterization of nanoparticles. Material and Methods: Ionic chelation method was used to prepare nanoparticles. The XTT assay was used to assess the cytotoxic activity of shRNA-VEGF and shRNA-VEGF loaded NP on the HeLa and NIH 3T3 cells. Results: IC50 values of shRNA-VEGF and NP including shRNA-VEGF were assessed. IC50 values of shRNA-VEGF and NP including shRNAVEGF were 0.89±0.010 and 0.52±0.004 μg/mL on HeLa cells. Bax amounts of control, shRNA-VEGF, and shRNA-VEGF loaded NP was calculated as 23.70±0.27, 34.64±0.36, and 39.46±0.54 ng/mg protein, respectively. The findings showed that cleaved caspase 3 amounts of control, shRNA-VEGF, and shRNA-VEGF containing NP was calculated as 711.70±4.40, 767.23±3.82, and 825.32±5.06 pg/mg protein. Conclusion: While producing no cytotoxicity in NIH 3T3 cells, shRNA-VEGF and shRNA-VEGF loaded NP substantially inhibited HeLa cell reproduction depending on the concentration. By using shRNA-VEGF and shRNA-VEGF loaded NP, the expression of pro-apoptotic Bax and cleaved caspase 3 proteins was considerably elevated.
