ATİKE TEKELİ, C SELİM İSBİR, ARZU ERGEN, NİLÜFER BOZKURT, UZAY GÖRMÜŞ, SİBEL BULGURCUOĞLU, SİNAN ARSAN
Advances in Molecular Medicine - 2007;3(2):77-83
Objectives: Cardiopulmonary bypass (CPB) induces a whole body inflammatory response and organ dysfunction eventually. This acute phase response after cardiac surgery induces the release of proinflammatory and antiinflammatory cytokines mainly interleukin (IL)-6, TNFa, IL-1b, IL-8 and IL-10. The aim of our study is to investigate whether IL8 polymorphism effect the plasma levels of IL-8 and also whether the levels of IL-8 have an effect on the outcome of the patients following surgery. Methods: Fourty seven patients underwent elective coronary artery bypass grafting (CABG) procedure with CPB. Genotyping for IL-8 (C1633T) was performed using a polymerase chain reaction-restriction fragment length polymorphism technique. Concentrations of IL-8 were measured before the induction, 4, 24 and 72 hours after the surgery by enzyme-linked immunosorbent assay. Results: IL8 levels although increased following CABG, did not show any significant difference between the carriers of 1633C and 1633T alleles at any time (p>0.05). Elevated IL8 levels at 24th postoperative hour were shown to be related with higher lactate levels, inotropic requirements in high doses and longer ventilation and ICU stay times. (p= 0.000, p=0.001, p=0.001 and p=0.012 respectively). Conclusion: These data suggest that IL-8 levels increase after CPB and associated with dreadfull postoperative course. IL-8 (C1633T) polymorphism however does not have any influence on the levels of IL-8.
