ZEYNEP APAYDIN, HAMİT YILDIZ, FATMA SAMİN, BELGİN ALAŞEHİRLİ
Experimental and Applied Medical Science - 2021;2(2):164-175
Thiols are antioxidant agents which belong to mercaptan group consisting of sulfur and carbon containing sulfhydryl ( SH). Thiol levels, in the case of elevated oxidative stress, are lowered by their use in neutralization of reactive oxygen molecules. It was aimed to investigate thiol and disulfide blood levels or thiol/disulfide ratio as a marker of total oxidant status which plays a role in the pathogenesis of inflammatory diseases that may cause respiratory failure. The study included 98 patients (58 males, 40 females) who have partial oxygen pressure (PaO2)<60 mmHg or partial carbon dioxide pressure (PaCO2)>45 mmHg in arterial blood in intensive care unit and 98 healthy volunteers. Total thiol and native thiol levels were measured by spectrophotometric method. Total thiol (270 ± 99.81), native thiol (203.90 ± 103.41) and disulfide (33.10 ± 12.42) levels of the patient group were significantly lower (p <0.001) than total thiol (423.62 ± 70.3), native thiol (307.13 ± 57.73) and disulfide (58.24 ± 27.21) of the control group levels. There is no significant difference between native thiol / total thiol, disulfide / total thiol and disulfide / native thiol ratios. This study may be the first study in the literature in terms of providing the diagnosis and follow up of dynamic thiol disulfide balance in patients with acute respiratory failure requiring intensive care support treatment. Thiol and disulfide blood levels or thiol / disulfide ratio can guide us as a prognostic test in acute respiratory failure patients.
