DAWON KANG, YOUNG-WOO CHO, WAN SEUNG KİM, JAEHEE HAN, SEA-YUONG JEON, DONG GU HUR, JUNG JE PARK, SEONG-Kİ AHN
The Journal of International Advanced Otology - 2013;9(1):75-81
Objective: The aim of this study was to examine the localization and expression of TREK-1, TREK-2 and TRAAK channels in rat medial vestibular nuclei by immunohistochemical staining, reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis. Materials and Methods: Twenty eight male Sprague-Dawley rats were used in this study. Antibodies for TREK-1, TREK-2 and TRAAK channels were used. Tissues containing medial vestibular nuclei were selectively for RT-PCR and Western blot analysis. Results: TREK-1, TREK-2 and TRAAK channels immunopositive neurons were distributed throughout the medial vestibular nuclei. RT-PCR data showed TREKs/TRAAK mRNAs were expressed in the medial vestibular nuclei. Western blot results also showed TREKs/TRAAK protein expression in the medial vestibular nuclei. Conclusion: This study may provide additional speculation into the role of K2p channels in the pathway between migraine and vestibular system.
