Güzide ŞATIR-BAŞARAN, Gülay SEZER, Merve YILDIRIM, Beyzanur AKTAŞ, Ahmed AMLEH, M. İhsan HAN
Journal of Research in Pharmacy - 2026;30(3):812-825
This study investigated the effect of a newly synthesized original molecule with a 1,2,4-triazole with a 2-fluorophenyl ring on miRNA. (S)-Naproxen was chosen as the starting molecule for synthesis. In molecular modeling studies, compound (4a) was used to control for the Bcl-2 apoptotic marker. The primary objective was to demonstrate that the MDA-MB-231 cell line exhibited growth inhibition in a dose-dependent manner following compound treatment. The results showed that compound (4a) inhibited the motility of MDA-MB-231 cells. Compound (4a) at concentrations of 75, 150, and 300 µM significantly reduced the viability of MDA-MB-231 cells compared to the control group. IC50 value of the compound (4a) for 48 h was 57.6 µM. In our study, for the first time, miR-133a-5p was significantly up-regulated in MDA-MB-231 breast cancer cells treated with compound (4a) at different concentrations compared to the control group. As such, miR-133a-5p may play a crucial role in tumorigenesis by targeting Bcl-2 in MDA-MB-231 cells and could serve as a biomarker for therapeutic targets in breast cancer treatment.
