HAMİT YILDIZ, MEHMET TAHİR HÜSUNET, İBRAHİM HALİL KENGER
Experimental and Applied Medical Science - 2022;3(1):298-309
Monoclonal antibodies are becoming increasingly important for molecular immunology research. It has also become key components in a wide variety of clinical laboratory diagnostic tests. The wide applications of serum analytes in the detection and identification of cell markers and pathogenic agents have arisen in large part due to the excellent specificity of these unique reagents. Furthermore, continuous culture of hybridoma cells producing these antibodies offers the potential for an unlimited source of reagents. In essence, the continuous supply feature provides standardization of both reagent and assay technique, compared to the very limited supply of polyclonal antibody reagents. Clearly, polyclonal and monoclonal antibodies have advantages and disadvantages in terms of production, cost, and general applications. As a result, monoclonal antibodies are produced only when necessary because their production is a time-consuming and laborious process, although highly rewarding. In this article, the production and application of monoclonal antibodies are illuminated to provide better understanding and formulate new ideas for clinicians and scientists alike.
