AVNİ SARAÇ, FERDA ALPASLAN PINARLI, FARUK GÜÇLÜ PINARLI, AYLAR POYRAZ, AYNUR OĞUZ, CEYDA KARADENİZ, ARZU OKUR, KEMALİ BAYKANER, ABDULLAH EKMEKÇİ, İREM DOĞAN
Turkish Journal of Oncology - 2019;34(4):215-222
OBJECTIVE The miR-17-92 cluster and miR-34a are short non-coding RNAs, which are important in tumorigenesis as they regulate numerous oncogenes and tumor suppressor genes. This study aimed to investigate the associations of mutations/polymorphisms of the miR-17-92 cluster and miR-34a coding sequences with high-grade central nervous system malignancies in pediatric patients. METHODS This study included 53 children with central nervous system malignancies and age- and gender-matched 27 healthy volunteers. Genomic DNAs were extracted from the paraffin-embedded tumor tissues (n=53) and peripheral blood samples (n=15) in the patient group and from the peripheral blood samples in the control group and were analyzed for mutations/polymorphisms of the miR-17-92 cluster and miR-34a coding sequences by DNA sequencing method. RESULTS There were no copy number alterations, amplifications, deletions, insertions, duplications, rearrangements, single nucleotide polymorphisms or mutations in the miR-17-92 cluster and miR-34a coding sequences of tumor tissue or blood samples in the patient group and of blood samples in the control group. CONCLUSION In children with high-grade brain tumors, no mutation was detected, leading to failures in regulations of miRNA coding DNA sequences of miR-17-92 and miR-34a. Further studies are needed to elucidate extremely complicated mechanisms underlying oncogenesis in high-grade central nervous system tumors.
