MEHWİSH IQTEDAR, NİMRA İLLYAS, MEHRAL ASLAM, ROHEENA ABDULLAH, AFSHAN KALEEM
The European Chemistry and Biotechnology Journal - 2025;2(4):40-52
New regimes of treatments and delivery systems are of great need today because of the increasing emergence of antimicrobial resist-ance among pathogens. In this study, initially liposomes were pre-pared comprising mycosynthesized silver nanoparticles in combina-tion with meropenem. As a result of this combination, nanosomes were designed. In vitro analysis of the prepared formulation was done to check its effectiveness against MDR Salmonella typhi. Extracellular synthesis of (AgNPs) silver-nanoparticles through Aspergillus fumig-atus BTCB01 was conducted. The particles were then characterized through by a zeta sizer, and they had particle size (299.6 nm), zeta potential (-2.64 mV) with a polydispersity value (0.4). Different liposo-mal formulations were prepared and characterized, each with different in size i.e. control liposomes had 318.7 nm, drug-encapsulated lipos-omes had 237.4 nm, silver-nanoparticles incorporated liposomes had 320.3 nm and Silver-nanoparticles incorporated drug liposomes had 215.3 nm size with different zeta potential and polydispersity index for each, i.e. -45.8 (PDI:0.3), -41.3 (PDI:0.3), -42.5 (PDI:0.4) and -39.6 mV (PDI:0.3), respectively, signifying a good stabile and homogene-ous solution. Meropenem encapsulated liposomes had (94%) encap-sulation efficiency, whereas Silver-nanoparticles incorporated drug liposomes had (86%) encapsulation efficiency. The release study of drugs through dialysis membranes graded in order of drug encapsu-lated liposomes > Silver-nanoparticles incorporated drug liposomes > control liposomes. In vitro analysis revealed that the antibacterial assay through the disk diffusion method revealed that drug-encapsu-lated liposomes showed a 1-fold increase in activity as compared to those which were incorporated with the silver nanoparticles. Conclusively, the addition of silver nanoparticles did not affect the stability of liposomes; however, they affected the encapsulation efficiency and drug release property of the delivery system.
