Okan AYKAÇ, Umut OKUR, Recep Ogün DEMİRCİ, Tuba ÖNER, Gülce TAŞKÖR ÖNEL, İrem BOZBEY MERDE
Anatolian Journal of Pharmaceutical Sciences - 2025;4(3):166-174
In this study, it was aimed to synthesize a new pyridazinone derivative compound with the potential to act as an acetylcholinesterase (AChE) inhibitor in the treatment of Alzheimer's disease and to encapsulate it into biodegradable poly(lactic-co-glycolic acid) (PLGA) microparticles together with lavender essential oil. The pyridazinone derivative compound was synthesized in five steps and its structure was elucidated by NMR and FTIR analyses. PLGA biopolymer was prepared by ring opening polymerization, and the pyridazinone-lavender oil mixture was encapsulated into microparticles by solvent evaporation method. Encapsulation efficiency was determined spectrophotometrically, and morphological analyses were performed by scanning electron microscopy (SEM). NMR and FTIR analyses showed that the target compound was successfully synthesized. According to GPC analysis, the average molecular weight of PLGA was found to be 3.019 Da and the PDI value was 1.408. SEM images showed that the microparticles were spherical, with smooth surface and in the size range of 5-20 µm. The encapsulation efficiency was determined as 78%. PLGA microparticles containing pyridazinone derivative compound and lavender oil can be considered as potential neuroprotective and anticholinesterase agents that can be used in Alzheimer's disease. This system, which provides controlled drug release thanks to its biodegradable structure, is recommended to be evaluated in advanced in vitro AChE/BuChE inhibition and cell culture studies.
