Basri Satılmış
Experimental Biomedical Research - 2025;8(4):281-290
Aim: Oleuropein (Ole) possesses a wide array of pharmacological benefits. Despite Ole's well -established anti-inflammatory and anti -tumor effects, there remains a gap in the research concerning its specific mechanism of action. We aimed to evaluate the effect s of Ole treatment on various characteristics, such as the viability of the cells, wound healing, colony formation, and the protein levels of the IL -6 / STAT3 pathway were evaluated in vitro in HepG2 HCC cells. Methods: Cell viability, wound healing, and c olony formation assays were performed. IL -6 levels measurement by ELISA, STAT3, and pSTAT3 protein expression measurement by Western blotting were performed in Ole -treated HepG2 cells. Results: Following the treatment of cells with Ole, there was a dose -dependent reduction in the viability of the cells and wound healing parameters. Colony formation was completely inhibited compared to control cells. Although IL -6 and STAT3 levels were strongly suppressed in Ole -treated cells compared to control cells, this difference did not achieve a statistical significance. Only pSTAT3 expression in Ole100 -treated cells was significantly downregulated compared to control cells. Conclusion: Based on our study, Ole treatment seems to have an impact on the characteristics o f HCC cells through its effect on increased cell death, reduced cell migration, and the downregulation of the IL -6 / STAT3 pathway.
