Esin KIRAY, İbrahim Seyfettin ÇELİK, Esen ÇAKMAK
Kocaeli Üniversitesi Sağlık Bilimleri Dergisi - 2026;12(1):33-40
Objective: In our study, we aimed to investigate the phytoconstitutions of the mastic gum extract obtained from the seeds of the Gundelia tournefortii L. (Kenger) plant, which has many beneficial activities on human health, in terms of antimicrobial, antibiofilm, antioxidant activities and cytotoxic activities on SK -MEL -30 and A2058 human melanoma cell lines. Methods: In the study where phytoconstitutions were determined by GC -MS analysis, the antimicrobial activity of the extract was determined by the well diffusion method. While the DPPH method was used in the antioxidant activity study, cytotoxic activity was investigated on SK -MEL -30 and A2058 human melanoma cell lines with MTT test kit. Results: Mastic extract showed low MIC value compared to control group with strong antimicrobial activity. It was determined that the mastic extract showed 62.18% biofilm inhibition on E. coli ATCC 25922 strain and 48.24% biofilm inhibition on P. aeruginosa ATCC 278533 strain at the highest concentration of 20 mg/ml in antibiofilm activity. In our study where we investigated antioxidant activity with the DPPH method, the IC50 value of the mastic extract was determined as 35.70 mg/mL. In terms of cytotoxic activity, it was determined that the extract showed a strong growth inhibitory effect with an IC50 value of 11.07 mug/ml in SK -MEL -30 cells and showed high cytotoxic effect at doses of 50 -1.8125 mg/mL in the A2058 cell line. Conclusion: These data contain valuable information as it is the first study to evaluate some biological activities of Kenger gum and will shed light on future studies.
