Raju ASIRVATHAM, Gowtham ANIRUDHAN, Dawn V TOMY, Saipriya SREEJAKUMARI, Vinod BALAKRISHNAN NAIR
İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi - 2026;14(2):238-250
Oxaliplatin, a potent chemotherapeutic agent, is clinically limited by adverse effects such as splenomegaly, hematological disturbances, hepatotoxicity, and peripheral neuropathy. The current study evaluated the protective efficacy of the aqueous extract of Indigofera tinctoria (AEIT) against oxaliplatin-induced toxicities in C57BL/6J mice. Thirty animals were divided into five groups: normal control, disease control, AEIT (500 mg/kg, p.o.), AEIT (1000 mg/kg, p.o.), and standard pregabalin (10 mg/kg, p.o.). Oxaliplatin (2.5 mg/kg, i.p.) was administered for four consecutive days across three weeks over a three-week period, while treatments continued from day 0-30. AEIT markedly alleviated body weight loss, splenomegaly, and hematological alterations. Biochemical analyses revealed restoration of antioxidant enzymes-glutathione peroxidase, superoxide dismutase, and catalase-accompanied by reduced levels of lipid peroxidation (malondialdehyde) and of serum aspartate aminotransferase. Histopathological evaluation confirmed the preservation of splenic and hepatic architecture. Additionally, behavioral assessments (hot-plate and tail-immersion tests) demonstrated significant analgesic and neuroprotective effects of AEIT (p < 0.001). Collectively, these findings indicate that AEIT mitigates oxaliplatin-induced oxidative stress, organ damage, and neuropathic complications. To the best of our knowledge, the present study provides early preclinical evidence supporting the protective potential of Indigofera tinctoria against oxaliplatin-induced splenic, hepatic, and neuropathic toxicities.
