AYŞE AKDOĞAN, NİHAL ŞEHKAR OKTAY
Acta Pharmaceutica Sciencia - 2025;63(1):236-247
The study aims to examine the effect of Methotrexate (MTX) treatment on rat spleen and investigate the antioxidant properties of quercetin (Que) and lipoic acid (LA) on MTX. Rats were divided into groups as Controls, MTX-given group, MTX+Que-given group, and MTX+LA-given group. At the end of the experiment, total protein, glutathione (GSH), lipid peroxidation (LPO), nitric oxide (NO), and total sialic acid (SA) levels, also superoxide dismutase (SOD), and catalase activities were determined in spleen homogenates. MTX increased oxidant parameters and both Que and LA effectively decreased LPO, SA, and NO levels. Administration of MTX increased GSH levels and decreased SOD activity, besides Que normalized GSH level and SOD activity, LA decreased GSH activity. Que and LA may be effective in recovering MTX-induced toxicity by decreasing oxidative stress and can be useful in normalizing the damage situation in rat spleen.
