Burcu BILTEKIN, Seyma DUMUR, Naile Fevziye MISIRLIOGLU, Hafize UZUN
International Journal of Medical Biochemistry - 2026;9(2):85-92
Objectives: Psoriasis is a chronic inflammatory disease characterized by epidermal dysregulation and increased oxidative stress. This study investigated the protective effects of boric acid (BA) in HaCaT keratinocytes exposed to lipopolysaccharide (LPS)-induced acute inflammatory redox stress using a simultaneous treatment protocol. Methods: HaCaT cells were assigned to control, LPS (10 µg/mL or 200 ng/mL), BA (20 or 100 µM), and simultaneous treatment (LPS + BA) groups. Cell viability was assessed by CCK-8 assay. Oxidative status was evaluated by malondialdehyde (MDA) and advanced oxidation protein products (AOPP), total sulfhydryl (TSH), and antioxidant parameters [superoxide dismutase (SOD) and catalase (CAT)]. Cell migration was analyzed by a wound healing assay. Results: LPS exposure did not cause overt cytotoxicity at 24 h but was associated with increased MDA and reduced CAT activity, indicating inflammatory oxidative stress. AOPP levels did not show a marked change under these acute conditions. Simultaneous BA administration maintained keratinocyte viability and attenuated LPS-associated lipid peroxidation, while partially restoring antioxidant defenses and improving wound closure. Conclusion: BA modulates oxidative stress markers and supports antioxidant defense and migratory capacity in LPS-stimulated keratinocytes. These findings support BA as a candidate redox-modulating compound that warrants validation in immune-competent and in vivo models relevant to psoriasis.
