Mehmet Fatih TEKIN, Dilek GÜL, Nurşah EKER, Adnan DAĞÇINAR, Yaşar BAYRI, Fatih BAYRAKLI, Beste Melek ATASOY, Süheyla Uyar BOZKURT
Balkan Medical Journal - 2026;43(4):196-205
Background: Accurate identification of medulloblastoma molecular subgroups is essential, particularly in settings lacking advanced genomic tools. Conventional markers, such as beta-catenin, present significant challenges in reliably detecting the WNT-activated subgroup (WNT-AG). Aims: To evaluate the utility of lymphoid enhancer-binding factor 1 (LEF1), Cyclin D1, and receptor tyrosine kinase-like orphan receptor 2 (ROR2) as immunohistochemical (IHC) markers for molecular subgroup classification and to assess their prognostic significance. Methods: IHC analysis was performed using LEF1, Cyclin D1, and ROR2. Two distinct LEF1 staining patterns were identified: nuclear (nLEF1) and punctate (pLEF1). A subset of 29 cases, selected based on predefined criteria, underwent EPIC array Methylation analysis. Findings were correlated with recurrence and survival outcomes.
