Elvan ALKAYA, Elif ÖNDER, Doğukan MUTLU, Nazlı ÇİL, Ahmet KOLUMAN, Şevki ARSLAN, Gülçin ABBAN METE
Van Medical Journal - 2026;33(1):112-119
Introduction: Hypochlorous acid (HOCl) is a physiological molecule synthesised by neutrophils during phagocytosis and plays an active role in the destruction of all microorganisms. In general, HOCl has been demonstrated to function as an antiseptic and an agent that induces apoptosis or cell necrosis in cells, depending on the ambient concentration. The aim of this study was to examine the impact of sodium hypochlorite (NaOCI) and zinc hypochlorite (ZnOCI), derived from diverse chlorine salts, on cellular proliferation and migration in keratinocyte and fibroblast cell lines in vitro. Materials And Methods: The effects of different concentrations of NaOCl and ZnOCl on keratinocyte (HaCaT) and fibroblast cell lines(L929) were compared using cell viability, colony formation and wound healing assays. In addition, the presence of apoptosis in the cells was determined using the TUNEL method. Results: The application of NaOCI and ZnOCI resulted in a substantial increase in colony formation in keratinocyte cell lines (HaCaT) in comparison to the control group, while this effect was not observed in fibroblast cell lines (L929). The results of the wound healing migration assay demonstrated that both NaOCI and ZnOCI were effective in the fibroblast cell line. However, when we compared the results of the two solutions in terms of wound healing and colony formation, ZnOCI was more effective than NaOCI in terms of colony formation in HaCaT cells and wound healing in L929 cells. NaOCI and ZnOCI reduced apoptosis in the HaCaT and L929 cell lines compared to the control group. NaOCI was more effective than ZnOCI in HaCaT cells. No difference was observed between the two applications in the L929 cell line. Conclusion: Further advanced research, including molecular studies, is required to understand the properties of NaOCI and ZnOCI.
