TUĞBA YILMAZ, DİLEK TÜZÜN, MURAT ŞAHIN, METİN KILINÇ
Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi - 2023;18(1):93-101
Objective: To examine the markers of oxidative stress in microvascular complications of diabetes, to examine the relationship of prolidase with oxidative stress indicators, to investigate the usability of prolidase as a predictive value. Material and Methods: 112 patients with type 2 diabetes mellitus (DM) and 44 healthy controls were included in the study. Detailed anamnesis of the patients was taken and physical examinations were performed. Fasting plasma glucose (FPG), hemoglobin A1c (HbA1C), creatinine, micrototal protein levels in spot urine were measured. Presence of diabetic retinopathy was investigated by fundus examination. Neurological examinations were performed for the presence of neuropathy. In addition, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA), nitric oxide (NO), serum prolidase activities were measured for oxidative stress in the morning blood sample of all patients. Results: Eighty (71.42%) patients with type 2 DM had complications, while 41 (28.58%) had no complications. Fasting plasma glucose was significantly higher in the diabetic complication group than in the other groups (p <0.001). The HbA1c level was significantly higher in the diabetic complication group than in the other groups (p <0.001). While antioxidant stress markers (CAT, SOD, GPx) were found to be lower in the diabetic complication group than in the other groups, oxidative stress markers (MDA, NO) were found to be higher in the complicated group than in the other groups (p<0.001, p<0.001, respectively). Prolidase level was found to be higher in the group with diabetic complications compared to the other groups (p<0.001). Conclusion: We think that prolidase activity will have a predictive value in determining microcomplications in diabetic patients, since serum prolidase activity and oxidative stress parameters were found to be statistically significantly increased in diabetic patients with complications compared to healthy controls and those with diabetes mellitus but without complications, and there was a positive correlation between prolidase and oxidative stress markers.
