Semra Şahin, Sa/fi ye Göçer
Journal of Biotechnology and Strategic Health Research - 2025;9(2):124-132
Aim Extended-spectrum beta-lactamases (ESBL) play an important role in the development of resistance to beta-lactam antibiotics in Gram-negative bacteria. ESBL-producing bacteria can cause outbreaks, especially in hospital infections, which can lead to inadequate treatment, prolonged hospital stay, and increased mortality rates. /T_h is study aimed to investigate the antibiotic resistance pro/fi les and ESBL production rates in Escherichia coli and Klebsiella spp. species isolated from various clinical samples in a tertiary care hospital Materials and MethodsA total of 315 bacterial isolates were included in the study between September 2022-January 2023. 213 of them were E.coli (67.6%) and 102 were Klebsiella spp. (32.4%). Antibiotic susceptibilities of the isolates; ampicillin, amoxicillin-clavulanate, piperacillin/tazobactam, cefuroxime, ce/fi xime, cefotaxime, gentamicin, amikacin, cipro/fl oxacin, trimethoprim-sulfamethoxazole, imipenem and meropenem were tested. ESBL production was evaluated with a double disk synergy test. /T_h e results were interpreted according to EUCAST criteria. Results ESBL positivity rate was determined as 35.2% in E. coli isolates and 52.9% in Klebsiella spp. isolates. /T_h is rate was found as 22% in pediatric patients and 42% in adult patients. When evaluated according to gender, ESBL positivity was found to be signifi cantly higher in male patients. According to clinical sample types, ESBL positivity was seen most in endotracheal aspirate and wound samples. According to the disk diff usion test, the highest resistance was detected against beta-lactam group antibiotics and the lowest resistance was detected against carbapenems. Conclusions ESBL product/i.doton continues to be a signifi cant resistance problem in hospital infections. /T_h is study draws attention to the importance of detecting ESBL-positive isolates with the double disk synergy test. Incorrect and unnecessary antibiotic use is one of the main risk factors for increasing ESBL positivity rates. /T_h erefore, early detection of ESBL-positive bacteria, isolation precautions and reconsideration of antibiotic policies are of great importance.
